Signalling
Part:BBa_K574009:Design
Designed by: Lei Wei Group: iGEM11_Tsinghua-A (2011-09-29)
3OC12HSL -> PoPS Receiver
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1048 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 386
Illegal AgeI site found at 583 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We want to design a system whose input is 3OC12HSL, thus we combine LasR coding sequence(BBa_C0079) with relevant promoter(BBa_R0079). Considering the possibility for various designs in the future(or in other words, the expandability), those regulated by pLas promoter depend on your need.
Source
We used BioBrick Standard Assembly. List as follows: BBa_K081005 BBa_C0079 BBa_B0015 BBa_R0079